Antigen-binding proteins comprising a linker which confers restricted conformational flexibility

Protéines se liant aux antigènes comprenant un liant conférant une flexibilité conformationnelle limitée

Antigenbindungsproteine mit einer Kopplung, die die strukturelle Beweglichkeit einschränkt

  • Inventors:
  • Assignees: Unilever Plc
  • Publication Date: May 24, 2000
  • Publication Number: EP-1002861-A1


Use of a polypeptide linker group, the amino acid sequence of which group confers restricted conformational flexibility, as a linking group to link binding units, preferably antigen binding units comprising heavy chain variable domains derived from an immunoglobulin naturally devoid of light chains, in a multivalent binding protein.




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Patent Citations (3)

    Publication numberPublication dateAssigneeTitle
    WO-9302198-A1February 04, 1993Technical Research Centre Of FinlandRecombinant secretable fusion proteins
    WO-9413804-A1June 23, 1994Medical Research CouncilMultivalent and multispecific binding proteins, their manufacture and use
    WO-9714719-A1April 24, 1997Unilever N.V., Unilever PlcA bifunctional or bivalent antibody fragment analogue

NO-Patent Citations (6)

    A. PLÜCKTHUN ET AL.: "New protein engineering approaches to multivalent and bispecific antibody fragments.", IMMUNOTECHNOLOGY, vol. 3, no. 2, June 1997 (1997-06-01), Amsterdam, NL, pages 83 - 105, XP004088466
    H. HOOGENBOOM: "Mix and match: Building manifold binding sites.", NATURE BIOTECHNOLOGY, vol. 15, no. 2, February 1997 (1997-02-01), New York, NY, USA, pages 125 - 126, XP002110046
    K. ALFTHAN ET AL.: "Properties of a single-chain antibody containing different linker peptides.", PROTEIN ENGINEERING, vol. 8, no. 7, July 1995 (1995-07-01), Oxford, GB, pages 725 - 731, XP002112077
    M. SRISODSUK ET AL.: "Role of the interdomain linker peptide of Trichoderma reesei cellobiohydrolase I in its interaction with crystalline cellulose.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 28, 5 October 1993 (1993-10-05), Baltimore, MD, USA, pages 20756 - 20761, XP002112074
    P. HOLLIGER ET AL.: "Specific killing of lymphoma cells by cytotoxic T-cells mediated by a bispecific diabody.", PROTEIN ENGINEERING, vol. 9, no. 3, March 1996 (1996-03-01), Oxford, GB, pages 299 - 305, XP002112075
    P. PORTOGHESE ET AL.: "Synthesis and opioid antagonist potencies of naltrexamine bivalent ligands with conformationally restricted spacers.", JOURNAL OF MEDICINAL CHEMISTRY, vol. 29, no. 9, September 1986 (1986-09-01), Washington, DC, USA, pages 1650 - 1653, XP002112076

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